Expression of thyroid hormone receptors in the heart of neonatus rats with maternal hyperthyroidism

doi:10.3969/j.issn.0529-1356.2013.04.019

Abstract

Abstract:

Objective To detect the expression of thyroid hormone receptors(TR), including TRα₁,TRα₂ and TRβ₁. Methods The animal model of hyperthyroidism was established (population and quantity:24 female Wistar rats). Took the neonatal rat hearts
of day 5, day 10 and day 15 after birth and stained paraffin sections with Masson staining. Detected expression of thyroid hormone receptors including TRα₁,TRα₂and TRβ₁ by real-time PCR. Results In the cardiac tissue, the TRα1 expression intensities were
higher than TRa₂ and TRβ₁. In addition, there was a peak in day 10 of neonatal rats both in the hyperthyroidism and the normal ones. In the hyperthyroidism rat, the TRα₁ expression intensities in day 5 and day 10 increased significantly,while it decreased
in day 15 in hyperthyroidism. There was no significant difference of TRa₂ expression in day 5, day 10 and day 15 between hyperthyroidism and normal. The TRβ₁ expression intensity decreased in day 5 in hyperthyroidsm, however, it increased in day 10. Conclusions
The maternal hyperthyroidism may influence the cardiac muscle of the neonatal rats by the different expression of thyroid hormone receptors. Due to the significant change of TRα₁, it may play a very important role in myocardial damage of hyperthyroidism.

Key words: Maternal hyperthyroidism, Thyroid hormone receptor (TR), Real-time PCR, Neonatal rat

CHEN Jing* LI Bin SONG Fang FENG Ruo-peng ZHAO Zi-wei. Expression of thyroid hormone receptors in the heart of neonatus rats with maternal hyperthyroidism[J]. AAS, 2013, 44(4 ): 546-549.

References

［1］Asahi T, Shimabukuro M, Oshiro Y, et al. Cilazapril prevents cardiac hypertrophy and postischemic myocardial dysfunction in hyperthyroid rats［J］. Thyroid, 2001,11(11):1009-1015.
［2］Ahmed OM, Abd ElTawab SM, Ahmed RG. Effects of experimentally induced maternal hypothyroidism and hyperthyroidism on the development of rat offspring: I. The development of the thyroid hormones-neurotransmitters and adenosinergic system interactions［J］. Int J Dev Neurosci, 2010,28(6):437-454.
［3］Li B, Chen J, Song F, et al. The model of hyperthyroidism in female rats［J］. Journal of Baotou Medical College, 2010, 26(6):1-2.(in Chinese)
     李斌, 陈晶, 宋芳, 等. 雌性甲亢大鼠模型的建立［J］. 包头医学院学报, 2010,26(6):1-2. 
［4］Klein I, Ojamaa K. Thyroid hormone and the cardiovascular systerm［J］. N Engl J Med, 2001, 344(7):501-509.
［5］Barber KJ, Franklyn JA, McCabe CJ, et al. The in vitro effects of triiodothyronine on epidermal growth factor-induced trophoblast function［J］.Clin Endocrinol Metab,2005,90(3):1655-1661.
［6］Liu ChR, Li LY, Liu B, et al. Expression of thyroid hormone receptor mRNA during the human brain development［J］. ActaAnatomica Sinica, 2003, 34(6):667-670.(in Chinese)
     刘春蓉,李兰英,刘奔,等. 人胎脑发育早期甲状腺激素受体mRNA的表达［J］.解剖学报,2003,34(6):667-670. 
［7］Luo M. The progress in thyroid hormone receptor［J］. Academic Journal of Second Military Medical University, 2001, 22
     (11):1059-1061.(in Chinese)
     罗敏. 甲状腺激素受体研究进展［J］.第二军医大学学报, 2001, 22(11):1059-1061. 
［8］Zhang J, Lazar M A. The mechanism of action of thyroid hormones［J］.Ann Rev Physiol,2000,62:439-466.
［9］Yen PM. Physiological and molecular basis of thyroid hormone action［J］.Physiol Rev,2001,81:1097-1142.
［10］Weatherman RSV, Fletterick RJ, Scanlan TS. Nuclear-receptor ligands and ligand-binding domains［J］.Ann Rev Biochem,1999,68:559-581.
［11］Mai W, Janier MF, Allioli N, et al. Thyroid hormone receptor is a molecular switch of cardiac function between fetal and postnatal life［J］.PNAS,2004,101(28):10332-10337.
［12］Wicktrom L, Johansson C, Salt C, et al.Abnormal heart rate and body temperature in mice lacking thyroid hormone recepto［J］.EMBO J,1998,17(2):455-461.
［13］Johansson C, Vennstrom B, Thoren P. Evidence that decreased heart rate in thyroid hormone receptor-alpha1-deficent mice is an intrinsic defect［J］.Am J Physiol,1998,275:R640.
［14］Li B, Chen J, Song F, et al. The differential expression of thyroid hormone receptors between hyperthyroidism and normal rats hearts in perinatal stage［J］. Journal of Anhui Agricultural Sciences, 2012, 40(21): 10916-10918.(in Chinese)
      李斌, 陈晶, 宋芳, 等. 甲状腺激素受体在围生期甲亢母鼠心脏中的差异表达［J］.安徽农业科学,2012,40(21): 10916-10918.

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